Tag: Mitotic

Free Download The Mitotic Spindle
English | 2025 | ISBN: 1071642235 | 283 Pages | PDF (True) | 34 MB
This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding know pitfalls.

Free Download Mitotic Exit
English | 2025 | ISBN: 1071642359 | 221 Pages | PDF (True) | 19 MB
This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications responsible for mitotic exit regulation; and ways to promote mitosis arrest in disease-associated conditions such as cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.